The Olympus FluoView 1000 Spectral-based Laser Scanning Confocal Microscope is configured on an Olympus IX81 automated inverted microscope platform with an XY automated stage and provides up to 6 lines of laser excitation. Laser excitation includes the blue diode laser (405 nm), the Argon gas laser (458 nm, 488 nm, and 514 nm), the green diode laser (559 nm) and the red Helium Neon gas laser (635 nm). High-resolution confocal fluorescence and/or reflection are detected through a single, variable pinhole aperture and are recorded using two high-sensitivity photomultiplier (PMT) detectors with with variable slits for spectral wavelength selection and one high-sensitivity PMT detector with band pass emission filters for wavelength selection. An independent PMT detector permits collection of brightfield, phase contrast, polarized light, and DIC contrast images.
The Olympus FluoView 1000 Spectral-based CLSM
Advanced imaging applications include:
- High-resolution confocal imaging in both fluorescence and reflected light modes
- Multi-channel confocal fluorescence imaging, including blue fluorescence (excitation 405 nm) through far red fluorescence (excitation 635 nm)
- 3D rendering and animation
- Co-localization analysis
- Live cell imaging and quantitative analyses over time
- FRAP and FRET analysis
- Fluorescence protein imaging, including CFP, GFP, YFP, RFP, and BiFC analysis
- Spectral imaging using variable slit detection with 1 nm wavelength selection
- Transmitted light imaging (brightfield, phase contrast, polarized light, DIC)