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Transmission Electron Microscopy (TEM)

The Center for Advanced Microscopy (CAM) offers full comprehensive transmission electron microscopy services for the biological sciences including plant and animal samples, bacteria, and pathology specimens.  In addition, full services are provided for materials science research such as polymers and plastics that require sample sectioning. 
TEM image of chloroplast by Dr. Alicia Withrow
TEM image of chloroplast by Dr. Alicia Withrow
The CAM also offers services for the physical sciences, including assistance using the JEOL 2200FS analyical materials TEM and a range of sample preparation equipment. The 2200FS is equipped with a unique “omega” energy filter to facilitate the clearest imaging in conventional mode and collection of energy loss spectra. See below for information about our two TEMs, related sample preparation equipment, and services.

The primary transmission electron microscope for biological sciences. There is no X-ray detector installed, but EDS can be performed on TEM grids using either the 2200FS TEM or the 7500F SEM.

Dr. Alicia Withrow operating the JEOL 1400 Flash TEMElectron gun

  • 100 kV (120 kV max)
  • LaBemitter
Sample holders and tilt
  • High-tilt holder
  • Four-position holder
  • tilt range +/- 70 degrees
Configuration and optics
  • lattice resolution 0.2 nm
  • high contrast pole piece
Detectors and cameras
  •  Matataki Flash sCMOS,  up to 30 fps 
Capabilities
  • Bright field or dark field imaging
  • Selected area electron diffraction (SAED)
Imaging features
  • Automated stitching/ montaging (Limitless Panorama)
  • Optical Microscope Correlation function in which an image from an optical microscope can be imported and overlaid on a TEM image to facilitate correlative microscopy.  

The primary transmission electron microscope for the physical sciences at the Center for Advanced Microscopy (CAM).

Electron gun

  • 200 kV  
  • Thermal field emitter (Schottky)
Sample holders and tilt
  • Single-tilt holder
  • Double-tilt holder
  • Analytical holder
  • Gatan cryo-holder
Configuration and optics
  • high tilt pole piece currently installed, UHR pole piece available
Detectors and cameras
  • Gata Rio CMOS camera with Digital Micrograph
  • Oxford X-ray detector / Inca
  • DE-20 direct electron camera (installed but not currently connected)
  • JEOL and Gatan ABF, ADF, and HAADF STEM detectors (not currently connected)
Capabilities
  • Bright field or dark field imaging
  • Selected area electron diffraction (SAED)
  • Covergent beam / nanobeam diffraction
  • Energy dispersive X-ray analysis (EDS)
  • Energy filtered imaging and electron loss spectroscopy (currently down)
  • STEM mode imaging (ABF, ADF, HAADF - currently down)
  • Cryo-TEM imaging
  • Electron tomography and low-dose imaging with SerialEM (currently down)
The JEOL 2200FS high resolution transmission electron microscope at the MSU Center for Advanced Microscopy
The JEOL 2200FS high resolution transmission electron microscope at the MSU Center for Advanced Microscopy, with Dr. Xudong Fan.
TEM images from the JEOL 2200FS (a) TEM image of a Mg-doped boron nano-wire. (b) Z-contrast STEM image showing Mg-doping site (c) diffraction pattern
Images taken on the JEOL 2200FS transmission electron microscope. (a) TEM image of a Mg-doped boron nano-wire. (b) Z-contrast STEM image showing Mg-doping site. (c) Diffraction pattern

For physical science TEM services contact Andy Elwood Madden, maddenan@msu.edu.

Enrollment in NSC-815 or NSC-816 TEM labs these labs is temporarily suspended). Please contact Andy Elwood Madden, maddenan@msu.edu. for more information.

The JEOL 2200FS 200 kV field emission TEM for physical sciences was funded by a National Science Foundation Major Research Instrumentation grant.

See our 'How to prepare samples' page for protocols, our sample preparation instrument page for related available resources, and our services page for more descriptions of available services.

For biological TEM services contact Dr. Alicia Withrow,  517-355-5004, pastorle@msu.edu.

For enrollment in NSC-810 Biological TEM Lab, contact Dr. Alicia Withrow,  517-355-5004, pastorle@msu.edu.  This is a limited enrollment course with specific enrollment procedures.  Please see our Courses page for more information.

See our 'How to prepare samples' page for protocols, our sample preparation instrument page for related available resources, and our services page for more descriptions of available services. For assitance with the 2200FS TEM, contact Dr. Andy Elwood Madden, maddenan@msu.edu.

TEM image of gold nanoparticlesImage of gold nanoparticles collected on the JEOL 2200FS